FIG. 4.

MMP7, MMP10, MMP9, and BSG mRNA expression after temporal PMA treatments. Cells were serum starved for 24 h and treated for an additional 4, 8, 12, or 24 h with vehicle control (DMSO) or 20 nM PMA. Messenger RNA expression was analyzed by real-time PCR at the indicated time points for (A) MMP7, (B) MMP10, (C) MMP9, and (D) BSG (i.e., Basigin) expression. Results are the means ± SEM for three individual measurements. A two-way ANOVA was performed; bars that do not share a letter designation are significantly different within a treatment group (P < 0.05).