Table 4.
Primers used in the present study (restriction sites are italics)
| Primers | Sequence (5’ → 3’) |
|---|---|
|
For plasmid construction |
|
| TA-U |
CCGGAATTCAAATGAAATAATACGGGAAGAGCG |
| TA-D |
CGGGGTACCCGGTAGCGATAATGTAAGAGGGTT |
| TB-U |
ACGCGTCGACGAACAGAACACAAAAGGAACAC |
| TB-D |
ACATGCATGCGAACCGCAATATTCAGAAACAC |
| SA-U |
CCGGAATTCCTTATAACGTGGGCCATGTCAT |
| SA-D |
CGCGGATCCCTAGTGACGTTGTCGTATTTGG |
| SB-U |
CGCGGATCCTCAACGAGAAATGTTGTGTAGC |
| SB-D |
CCCAAGCTTACATATGCTCATCGGGAAAACC |
| Kan-U |
CGCGGATCCCAGCTGAAGCTTCGTACGC |
| Kan-D |
CGCGGATCCGCATAGGCCACTAGTGGATCTG |
|
For PCR verification |
|
| YT-U |
TCTTGTCTGTCTGCTTCTTCACTGT |
| YT-D |
AAAGAGTGTGAAGTGACGGCTATG |
| YS-U |
CACACTCCGTTCTTAGTGGTTGTT |
| YS-D |
ATCCACCGTAGAACCCAAAGCATT |
| K-U |
CTTGCTAGGATACAGTTCTCACATCA |
| K-D |
CGCATCAACCAAACCGTTATTCATTC |
| Z-U |
CCCACACACCATAGCTTCA |
| Z-D | AGCTTGCAAATTAAAGCCTT |