5’ end-centered expression profiling using Cap-analysis gene expression (CAGE) and next-generation sequencing

. Author manuscript; available in PMC: 2014 Jul 11.

Published in final edited form as: Nat Protoc. 2012 Feb 23;7(3):542–561. doi: 10.1038/nprot.2012.005

Cycle number	Denature	Anneal/Extend	Hold
1	98°C, 30 sec	-	-
2-8/10/12	98°C, 10 sec	60°C, 10 sec	-
9/11/13	-	-	4°C