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. 2014 Jul 11;9(7):e100380. doi: 10.1371/journal.pone.0100380

Figure 1. HPLC profile, methylation analysis result and 1D NMR spectrums of GSP-2.

Figure 1

A: HPLC profile of GSP-2. Samples (2 mg/mL, 10 µL) were analyzed on an Agilent 1100 system equipped with an ELSD detector and TSK GMPWXL gel filtration columns (7.8×300 mm×2), with 20 mM CH3COONH4 as mobile phase at 0.6 mL/min and column temperature at 40°C. Commercially available T-series dextrans (MW 2000, 670, 410, 270, 150, 80, 50, 12, 5 and 1 kD). B: Methylation analysis result of GSP-2. GC-MS tests for methylation analysis were measured with a DB-5 column (30 m×0.25 mm×0.25 µm), and at temperatures programmed from 170–225 °C at 2 °C/min and then hold on 10 min, in the figure, a: T-Glcp, b: 1,3-linked Glcp, c: 1,4-linked Glcp, d: 1,6-linked Glcp, e: 1,3,6-linked Glcp. C-E: 1D NMR (1H, 13C, DEPT) spectra of GSP-2 in D2O with TMS as external standard, obtained on a Bruker AM 500 spectrometer with a dual probe in the FT mode at room temperature.