Figure 3. Stimulating effect of GSP-2 on the proliferation of the mouse splenocytic B and T cells.

Freshly fractionated splenocyte, splenic B (solid bars) and T (open bars) cells were stimulated with, or without (Medium), dextran or GSP (30 µg/ml). LPS (2 µg/ml) and ConA (2 µg/ml) were included as controls. In a parallel experiment, mouse splenocytes were stimulated with, or without (Medium), GSP (30 µg/ml), dextran (30 µg/ml) or LPS (10 µg/ml) in triplicate wells in the presence, or absence, of PMB. ³H-TdR was added to the cultures for the last 8 hrs of incubation and then ³H-TdR incorporation (CPM) of each well counted. A: Parallel experiment to exclude the influence of the endotoxin contamination; B: Stimulating effect of GSP-2 to the mouse splenocytic B and T cells. All results are presented as mean ± SEM, *, P<0.05; **, P<0.01; ***, P<0.001 for difference from culture without treatment. (n = 9, repeated 3 times).