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. 2014 Jul 11;9(7):e100822. doi: 10.1371/journal.pone.0100822

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© 2014 Schlederer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A Western Blot of p-STAT5^Tyr694 expression in Stat5ab^+/+ (n = 2), Stat5ab^+/Δ (n = 2) and Stat5ab^Δ/Δ (n = 2) livers of GH treated and control mice. HSC70 is shown as loading control. B IHC of p-STAT5^Tyr694 in sections of the livers (n = 3) used in A. Red, AEC; blue, hematoxylin. Size bar: 100 µm. High magnifications (60x) are shown in insets. C Quantification of p-STAT5^Tyr694 levels from Western blots (grey) by densitometric scanning and IHC (magenta) using the HistoQuest software (Stat5ab^+/+, control; number of cells n = 3276, GH; number of cells n = 2286; Stat5ab^+/Δ control; number of cells n = 2577, GH; number of cells n = 3549; Stat5ab^Δ/Δ control; number of cells n = 3299, GH; number of cells n = 2200). The highest arbitrary expression values from A and B were set to 100 for direct comparison. D Western blot of p-STAT3^Tyr705 expression in Stat5ab^+/+ (n = 2), Stat5ab^+/Δ (n = 2) and Stat5ab^Δ/Δ (n = 2) livers of GH treated and control mice. HSC70 is shown as loading control. E IHC of p-STAT3 ^Tyr705 in sections of the livers (n = 3) used in D. Red, AEC; blue, hematoxylin. Size bar: 100 µm. High magnifications are shown in insets (60x). F Quantification of p-STAT3^Tyr705 levels from Western blots (grey) by densitometric scanning and IHC (magenta) using the HistoQuest software (Stat5ab^+/+, control; number of cells n = 2634, GH; number of cells n = 2286; Stat5ab^+/Δ control; number of cells n = 22230, GH n = 2562; Stat5ab^Δ/Δ control; number of cells n = 2414, GH n = 2716). The highest arbitrary expression values from A and B were set to 100 for direct comparison.