Figure 2. Biofilm formation by individual brp gene mutants.

Biofilm formation was assessed qualitatively and quantitatively for opaque, rugose, and translucent control variants, the 4 brp mutant strains, and the complemented mutants. A. Following ON growth with shaking, 3 cultures per strain were assessed for pellicle formation and a representative is pictured. Pellicle thickness was scored qualitatively as — (no pellicle), + (thin pellicle), ++ (pellicle), or +++ (thick pellicle). B. Biofilm assays were performed on at least 6 independent culture replicates of each statically grown strain and OD₅₇₀ values, which correspond to the amount of crystal violet staining of biofilm material, were averaged. Averages ± standard deviations (SD) are pictured here. Asterisk denotes p<0.001 versus KG3(R).