Table 3. Primers used for non-polar mutagenesis & complementation experiments.
| Primer | Sequence (5′→3′)1 |
| Npm01 | AAAAAGAATTCCGACGAGGCCTTCTGTGGTG |
| Npm02 | AAAAAGATATCCGAAGTGAGGCGTCCCGGTAG |
| Npm03 | AAAAACTCGAGGTAAGAGGCATACCTTCC |
| Npm04 | AAAAAGAATTCTACGATCTGCCACTCTAC |
| Del_brpA_A | AAAGAATTCACGCCACTTTGGTAGGAG |
| Del_brpA_B | GTCCATTTA CCCGGG ACAAGCCCGTGTTTAAAGGCTTTACTGG |
| Del_brpA_C | GCTTGT CCCGGG TAAATGGACTCATGTGAGCGAATCAATCC |
| Del_brpA_D | AAAGGATCCCAGATGCGTGCCCAGATTAC |
| Del_brpJ_A | AAAGAATTCGCGCCAATTTCAGGCAAGGTAG |
| Del_brpJ_B | CTTCAAT CCCGGG GATCCAGCTTAGGCTTTGTACCCATCG |
| Del_brpJ_C | CTGGATC CCCGGG ATTGAAGCTCATGGGATAACCCAGAC |
| Del_brpJ_D | AAATCTAGAAAGCATTTGGCGGTGGTGTAC |
| brpA-For | AAACTCGAGCCCACCCACACGGGTAACAAAC |
| brpA-Rev | AAAAAGCTTAACGACACTCAAGGGCGGTAGC |
| Npm11 | AAAAATCTAGATGATTAAGCACGCTGAGC |
| Npm12 | AAAAACTGCAGCAGAAAAGGAGAGAGCTC |
| Npm132 | AAAAAGGATCCCTTCGCCAGTAACGAGGTCG |
| brpJ-For | AAACCCGGGAAGATTGGCGCTCAGCAGCC |
| brpJ-Rev | AAATCTAGAACTGTCATGACGCAAATGAC |
| brpK-For | AAAAATCTAGAGCAATGGCGATGTCTTGCTG |
| brpIJK-Rev | AAAAACCCGGGCTAAAGACACCAGGGACGAG |
1
Restriction enzyme sites are underlined. Homologous sequence used in SOE is italicized. SmaI sites are in bold.
2
From Grau, et al. 2008 [7].