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. 2014 Jul 11;9(7):e101914. doi: 10.1371/journal.pone.0101914

Figure 8. Heat stress treatment of mutant lines.

Figure 8

(A) Germination rates for the BT assay. Seeds were treated at 45°C for 5 h immediately after vernalization at 4°C, and germination rates were measured after an additional 2 d. Survival rates for the BT assay at 45°C for 30 min (B), ATSR assay at 45°C for 150 min (C) and ATLR assay at 45°C for 1 h (D). 7-d-old seedlings were used in analyses and survival rates of each sample was measured at 7 dafter heat stress. Data were collected from three replicates, each with 30 seeds or 20 seedlings. * and ** indicate significant differences compared with WT plants for P<0.05 and P<0.01, respectively, as determined by a t-test.