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. 2014 Jun 27;15:73. doi: 10.1186/1471-2350-15-73

Figure 5.

Figure 5

Extracellular ATP enhances monocyte expression of FAM134B. (A) Location of the rs257174 SNP-cluster with reference to the two common splice variants of FAM134B. The FAM134B transcript exists in two isoforms, a long isoform (~9 kbp down-stream of the rs257174 SNP-cluster), and a less abundant short isoform (more than 100 kbp distal from the rs257174 SNP-cluster). (B) CD14+ monocytes were isolated from 3 separate individuals and exposed to variable doses of ATP (0, 0.2 and 1 mM). After 2 h incubation, expression of FAM134B was measured by RT-PCR and normalized by the 2-∆c(t) method using HPRT as the housekeeping reference gene. FAM134B expression in monocytes was significantly and dose-dependently up-regulated in response to extracellular ATP treatment. Differences between the 3 treatments were estimated using repeated measures ANOVA (P = 0.0089) with Tukey’s post-hoc comparison test on ∆c(t) values. Error bars represent standard deviation across biological replicates.