Table 2. Analysis of PPRV neutralization activity in sera from vaccinated and non vaccinated sheep.
Neutralization titera | |||||||||
Treatmentb | Sheep # | D0c | D21d | D42d | D3PChe | D5PChe | D7PChe | D11PChe | D13PChe |
PBS | 1 | <2 | <2 | <2 | <2 | 16 | 40 | 160 | 200 |
2 | <2 | <2 | <2 | <2 | 16 | 80 | 80 | 200 | |
3 | <2 | <2 | <2 | <2 | 16 | 80 | 160 | 200 | |
4 | <2 | <2 | <2 | <2 | 16 | 80 | 320 | 400 | |
Ad5 | 5 | <2 | <2 | <2 | <2 | 16 | 80 | 160 | 200 |
6 | <2 | <2 | <2 | <2 | 4 | 80 | 80 | 400 | |
7 | <2 | <2 | <2 | <2 | 16 | 160 | 160 | 400 | |
8 | <2 | <2 | <2 | <2 | 16 | 20 | 80 | 100 | |
Ad5-PPRV-F | 9 | <2 | 4 | 4 | 8 | 64 | 160 | 320 | 400 |
10 | <2 | 4 | 8 | 8 | 32 | 160 | 160 | 200 | |
11 | <2 | 4 | 8 | 8 | 64 | 160 | 320 | 400 | |
12 | <2 | 4 | 4 | 64 | 64 | 160 | 320 | 400 | |
Ad5-PPRV-H | 13 | <2 | 4 | 4 | 16 | 64 | 160 | 320 | 400 |
14 | <2 | 2 | 4 | 4 | 8 | 20 | 80 | 800 | |
15 | <2 | <2 | 16 | 16 | 64 | 160 | 320 | 800 | |
16 | <2 | 2 | 4 | 4 | 64 | 160 | 320 | 800 |
SN titers were determined against Nigeria 75/1 PPRV strain and expressed as the reciprocal of the last dilution of serum that neutralized 50% of the virus-specific cytopathic effect in flat bottom 96 well plates.
Intramuscular inoculations of PBS, Ad5 or vaccine vectors as described in the Material and Methods section (See Table 1).
Sera obtained from uninfected sheep.
Sera obtained from vaccinated sheep after 21 days post first immunization and 21 days post second immunization (42 days post first immunization).
Sera obtained from infected sheep after days 3,5,7,11,13 post challenge.