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. 2014 Jul 11;9(7):e101226. doi: 10.1371/journal.pone.0101226

Table 2. Analysis of PPRV neutralization activity in sera from vaccinated and non vaccinated sheep.

Neutralization titera
Treatmentb Sheep # D0c D21d D42d D3PChe D5PChe D7PChe D11PChe D13PChe
PBS 1 <2 <2 <2 <2 16 40 160 200
2 <2 <2 <2 <2 16 80 80 200
3 <2 <2 <2 <2 16 80 160 200
4 <2 <2 <2 <2 16 80 320 400
Ad5 5 <2 <2 <2 <2 16 80 160 200
6 <2 <2 <2 <2 4 80 80 400
7 <2 <2 <2 <2 16 160 160 400
8 <2 <2 <2 <2 16 20 80 100
Ad5-PPRV-F 9 <2 4 4 8 64 160 320 400
10 <2 4 8 8 32 160 160 200
11 <2 4 8 8 64 160 320 400
12 <2 4 4 64 64 160 320 400
Ad5-PPRV-H 13 <2 4 4 16 64 160 320 400
14 <2 2 4 4 8 20 80 800
15 <2 <2 16 16 64 160 320 800
16 <2 2 4 4 64 160 320 800
a

SN titers were determined against Nigeria 75/1 PPRV strain and expressed as the reciprocal of the last dilution of serum that neutralized 50% of the virus-specific cytopathic effect in flat bottom 96 well plates.

b

Intramuscular inoculations of PBS, Ad5 or vaccine vectors as described in the Material and Methods section (See Table 1).

c

Sera obtained from uninfected sheep.

d

Sera obtained from vaccinated sheep after 21 days post first immunization and 21 days post second immunization (42 days post first immunization).

e

Sera obtained from infected sheep after days 3,5,7,11,13 post challenge.