Table 3. ELISA determination of PPRV N antigen seroconversion in vaccinated and non vaccinated sheep.
Treatmenta | Sheep # | D0b | D42c | D3PCd | D5PCd | D7PCd | D11PCd | D13PCd |
PBS | 1 | - | - | - | + | + | + | + |
2 | - | - | - | + | + | + | + | |
3 | - | - | - | + | + | + | + | |
4 | - | - | - | + | + | + | + | |
Ad5 | 5 | - | - | - | + | + | + | + |
6 | - | - | - | de | + | + | + | |
7 | - | - | - | + | + | + | + | |
8 | - | - | - | + | + | + | + | |
Ad5-PPRV-F | 9 | - | - | - | + | + | + | + |
10 | - | - | - | + | + | + | + | |
11 | - | - | - | de | + | + | + | |
12 | - | - | - | de | + | + | + | |
Ad5-PPRV-H | 13 | - | - | - | + | + | + | + |
14 | - | - | - | - | + | + | + | |
15 | - | - | - | + | + | + | + | |
16 | - | - | - | + | + | + | + |
Intramuscular inoculations of PBS, Ad5 or vaccine vectors as described in the Material and Methods section (See Table 1).
Sera obtained from:
Non-vaccinated sheep at day 0.
sheep at 21 days post second immunization (42 days post first immunization) or from.
PPRV challenged animals at the indicated days.
No clear positive nor negative PPRV detection.