Table 4. ELISA detection of PPRV in vaccinated and non-vaccinated sheep.
Treatmenta | Sheep # | D42b | D3PCc | D5PCc | D7PCc | D11PCc | D13PCc | ||||||||||||
Swab | Cd | Ne | Of | Cd | Ne | Of | Cd | Ne | Of | Cd | Ne | Of | Cd | Ne | Of | Cd | Ne | Of | |
PBS | 1 | - | - | - | - | - | - | - | + | + | + | + | + | - | - | + | - | - | - |
2 | - | - | - | - | - | - | - | - | + | - | + | + | - | - | - | - | - | - | |
3 | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | |
4 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
Ad5 | 5 | - | - | - | - | - | - | - | + | - | + | + | + | + | + | - | + | - | - |
6 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | ||
7 | - | - | - | - | - | - | - | + | + | + | + | + | + | - | - | - | - | - | |
8 | - | - | - | - | - | - | + | - | + | + | + | + | + | - | + | - | - | - | |
Ad5-PPRV-F | 9 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
10 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
11 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
12 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
Ad5-PPRV-H | 13 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
14 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
15 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
16 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Intramuscular inoculations of PBS, Ad5 or vaccine vectors as described in the Material and Methods section (See Table 1).
Sera obtained from non-infected sheep at 21 days post second immunization (42 days post first immunization) or from.
PPRV challenged animals at the indicated days post challenge.
Conjunctive swabs.
Nasal swabs.
Oral swabs.