Table 2. Internal transcribed spacer fragment sizes determined by capillary gel electrophoresis for clinical isolates, including common fragments and unique lengths that characterise a species.
Species | No. of isolates | No. of CGE patterns | Single peak (%) | Common fragment sizes in bp (frequency) | Signature (unique) fragment lengths in bp |
M. chelonae | 40 | 1 | 100 | 384(14)/385(26) | 384/385 |
M. abscessus | 31 | 2 | 100 | 388.0(29)a | 388.0/393 |
M. massiliense | 17 | 1 | 100 | 388.5(11)a/389.0(6) | 388.5/389 |
M. fortuitum | 64 | 27 | 17 | 429(22)/462(7)/475(36) | 415/420/421/430/431/434/435/438/439/455/456/457/458/460/462/463/473/475/479/489/491/529/532/534 |
M. mucogenicum | 13 | 6 | 92 | 522(8) | 332/372/516/518/521/522 |
M. flavescens | 5 | 2 | 80 | 468(4) | 468/477/506 |
M. elephantis | 3 | 3 | 33 | 429(2) | 463/480/498 |
M. immunogenum | 1 | 1 | 100 | 400 | 400 |
M. moriokaense | 1 | 1 | 100 | 478b | |
M. neoaurum | 1 | 1 | 0 | 490/505 | 505 |
M. phlei | 1 | 1 | 100 | 461 | 461 |
M. septicum | 1 | 1 | 0 | 467/493 | 467/493 |
Rounding to 0.5 base pairs allowed for reliable separation of M. abscessus and M. massiliense.
M. moriokaense could not be separated from two isolates of M. fortuitum with indistinguishable electropherograms displaying single peaks at 478 bp.