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. 2014 Jul 11;9(7):e102352. doi: 10.1371/journal.pone.0102352

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© 2014 Boczek et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cells expressing mitoSypHer were loaded with 10 µM Fura-2 for 1 h and simultaneous changes in Fura-2/mitoSypHer fluorescence were recorded in single cells. The traces show the mean response of (Ca²⁺)_c (upper panel) and pH_mito (lower panel) in n = 11 cells for C, _2, _3 lines following repetitive 59 mM KCl stimulation and recovery.