Skip to main content
. 2014 Jul 11;9(7):e102352. doi: 10.1371/journal.pone.0102352

Figure 5. The response of mitochondrial pH to the inhibition of an efflux and the release component of (Ca2+)c elevations.

Figure 5

(A) The average effect of extracellular Ca2+ removal and its subsequent restitution on (Ca2+)c and pHmito changes in n = 18, n = 12, n = 21 cells for C, _2, _3 lines respectively, pretreated for 20 min with thapsigargin and 2-APB. The insets show SERCA-independent (Ca2+)c clearance (upper) and pHmito recovery (lower). (B) The average effect of cadmium (VDCCs inhibitor) in n = 29, n = 19, n = 22 cells for C, _2, _3 lines respectively, on KCl-evoked (Ca2+)c influx and concomitant pHmito changes. (C) The average effect of 5 mM La3+ in n = 16 cells for C, _2, _3 lines showing delay in (Ca2+)c clearance and pHmito alkalization under low extracellular Na+. (D) The average effect of extracellular alkaline pH (9.0) followed by pH return to 7.4 in n = 20, n = 12, n = 20 cells for C, _2, _3 lines respectively, on (Ca2+)c elevations and pHmito changes.