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. 2014 Jul 11;9(7):e102352. doi: 10.1371/journal.pone.0102352

Figure 6. Changes in (Ca2+)c and ΔpH are reproduced in transiently transfected cells.

Figure 6

(A) Immunodetection of PMCA2 or PMCA3 in PC12 cells transiently transfected with phosphothioate oligodeoxynucleotide probes. (B) Densitometric analysis of PMCAs showing ∼75% knock-down of target genes. The results are presented as arbitrary units (AU) obtained after normalization to endogenous GAPDH level. The dotted line shows the value for untransfected cells (control cells). * P<0.05 PMCA-downregulated cells vs. control cells. (C) The effect of transient PMCAs silencing on (Ca2+)c in n = 17 cells for each line without or (D) with the presence of thapsigargin and 2-APB in n = 20 cells for each line. (E) corresponding changes in ΔpH without or (F) with the presence of thapsigargin and 2-APB.