Table 1. Antisense phosphothioate oligodeoxynucleotides used for PMCA2 or PMCA3 knock-down in a model of transient transfection.
Primer | Sequence |
PMCA2-1 | 5′-C*CTTGGGCCGTGGCACATCCTTCATTGCT*C-3′ |
PMCA2-2 | 5′-G*GTGAGCTTGCCCTGAAGCACCGACTTCT*C-3′ |
PMCA2-3 | 5′-C*CAGCAGGCCACACTCTGTCTTGTTGCCC*A-3′ |
PMCA3-1 | 5′-C*TGCCCATAGATCTGCCTGCGTTTCTCCAAGT*C-3 |
PMCA3-2 | 5′-A*CCCGGCCATCCACAACGAAGGTCTCAATCAC*A-3′ |
PMCA3-3 | 5′-T*TACCATGTCATCCCGGTCCCGAGGACGAAAT*C-3′ |
Mismatch | 5′- TGTGAATCTGTTAGCCTTAACCTTAAGTTC-3′ |