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. 2014 Jul 11;9(7):e102246. doi: 10.1371/journal.pone.0102246

Figure 2. SYTOx green assay to visualize pore formation mediated by nisin.

Figure 2

The NZ9000Erm strain (black line), NZ9000NisI strain (blue line) and the NZ9000NisIΔ22 strain (red line) were grown and incubated with the SYTOX green dye. The fluorescence signal was monitored online using a fluorolog (Horiba III). After 400 seconds a stable baseline was reached and nisin was added (A) 10 nM (B) 30 nM and (C) 1000 nM. The addition of nisin is indicated with an arrow. The rapid increase of the fluorescence signal indicated pore formation. The data are representatives of at least three independent measurements.