Figure 2. Interrupted stress has greater impact on body weight and adrenal gland weight.

A) Weight gain over time in the daily stress group compared to the daily control group was measured. There was a significant Day x treatment pattern (2-way repeated measures ANOVA). However, body weight on any individual day was not significantly different between groups in post hoc comparisons after correction for multiple comparisons. B) Interrupted stress led to a transient difference in body weight that recovered during the interruption, but reappeared after the resumption of stress. C) When measured one day after the last stress or control session, daily stress did not have a significant effect on body weight, while interrupted stress significantly decreased body weight. D) Adrenal gland weight was increased by daily or by interrupted stress (normalized to body weight displayed here). The effect of interrupted stress on adrenal gland weight was significantly greater than daily stress. * indicates p<0.05 in post hoc tests after significance in a two-way repeated measured ANOVA (B) or two-way ANOVA (C, D). # indicates significant Pattern x Day interaction in a two-way ANOVA.