Figure 6. Interrupted restraint has greater impact on anxiety-like behavior.

Exploration in the elevated plus maze (EPM) was measured. A) There was no significant difference in total arm entries between the 9 day daily and interrupted restraint groups, and their controls. B) Daily and interrupted restraint both led to decreased amount of time spent in the open arms. The effect of interrupted restraint stress on time in the open arms was significantly greater than the effects of daily restraint stress. C) There was no significant effect of one or two day stress exposure on total arm entries compared to controls. D) There was no significant effect of one or two day stress exposure on time in open arms compared to controls. * indicates p<0.05 in post hoc tests after two-way ANOVA.