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. 2014 Jun;37(2):350–359. doi: 10.1590/s1415-47572014005000002

Table 1.

OCT 1 Multiplex PCR primers.

Primer name Length Tm Nucleotide sequence Amplicon size
Exon 1 24 65.9 5′ - TGCTGAGCCATCATGCCCACCGTG - 3′ 255
21 62.8 5′ - GGACACAGCCAGACACCCACG - 3′
Exon 2 24 59.6 5′ - CTCTTGCCGTGGTATGACTGGCAG - 3′ 162
23 57.9 5′ - CAGAGGGGCTTACCTGGACTGG - 3′
Exon 3 25 58.1 5′ - CCTCCATGTCTCCTTCTCTCTGAAG - 3′ 207
25 57.2 5′ - CTGGCCTCATCCCCATGATAATTAC - 3′
Exon 4 24 61.3 5′ - CCCGCATAACGTCCACACCTCCTG - 5′ 222
23 60.3 5′ - GTAGGCAGGAGGAAGGGCCTCAC -3′
Exons 5 and 6 24 57.4 5′ - GATAGTGATGAGTGGTGTTCGCAG - 3′ 503
21 62.7 5′ - GCGAGCGTGCTGATTCTGCCT - 3′
Exon 7 25 59.3 5′ - GACTTGAAACCTCCTCTTGGCTCAG - 3′ 298
25 64.2 5′ - TTCCCCACACTTCGATTGCCTGGGA - 3′
Exon 8 25 67.6 5′ - GAAGCCCCCATCCACCACCCACACC - 3′ 181
25 63.4 5′ - GGCTACCCCTGTTCCATGCACTCAC - 3′
Exon 9 22 62.4 5′ - ATTGCATGGGCAACGGATGGCT - 3′ 615
25 67.8 5′ - CCATGCTGAGCCCACTGCCGAGCTG - 3′
Exon 10 23 60.6 5′ - TTCCTCTCTTTGGCTGGCTGTGA - 3′ 621
24 60.5 5′ - ACTCCAGCAAACCTTGCTCTCTGT - 3′
Exon 11 25 58.9 5′ - TGCCCTTTTCTTCTTTGCTGTTTGC - 3′ 460
25 60.8 5′ - AGCACCAACAGCTTTCCCTAGATCG - 3′
Intron 9 25 58.8 5′ - GAGTAGGAGGGGTTAATAGAGAGAG - 3′ 236
27 65.7 5′ - GTAGCTGAGACTACATGCATGCACCAC - 3′