Table 4.
Greenhouse experiment with the inoculation of L. corniculatus plants with rhizobial isolates or reference strains that are able to nodulate both L. corniculatus and D. incanum plants.
| Treatment | Number of nodules per plant | Nodules dry matter (mg plant −1) | Root dry matter (mg plant −1) | Shoot dry matter (mg plant −1) | Nitrogen content (mg plant −1) |
|---|---|---|---|---|---|
| N+ * | - | - | 1065.5 ± 193.5 a | 1008.5 ± 85.8 a | 5.63 ± 0.4 c |
| SEMIA 816 | 538.5 ± 79.9 a | 109.5 ± 14.1 a | 627.25 ± 97.1 b | 1120.25 ± 134.0 a | 14.22 ± 2.3 a |
| EEL 698 | 290.25 ± 93.1 b | 71.5 ± 29.8 b | 365 ± 70.9 c | 875 ± 118.3 a | 10.1 ± 2.5 b |
| UFRGS Ls1 | 231 ± 97.2 b | 51.5 ± 6.2 c | 189.25 ± 31.8 c | 332.5 ± 93.0 b | 2.63 ± 1.1 d |
| UFRGS Lu2 | 280.5 ± 79.8 b | 83.5 ± 33.0 b | 335.25 ± 72.8 c | 992.75 115.3 a | 12.53 ± 0.6 a |
| UFRGS Lu13 | 334 ± 32.6 b | 53.25 ± 13.3 c | 221 ± 54.8 c | 454.75 ± 81.5 b | 2.48 ± 0.6 d |
| UFRGS Di9 | 143.75 ± 25.6 c | 40.75 ± 6.1 c | 292.25 ± 92.7 c | 265 ± 83.8 b | 1.31 ± 0.6 d |
| UFRGS Lc336 | 155 ± 63.7 c | 36 ± 16.2 c | 182.5 ± 67.9 c | 337.75 ± 68.2 b | 2.41 ± 0.7 d |
| N- * | - | - | 304.75 ± 64.1 c | 207.25 ± 65.3 b | 0.57 ± 0.1 d |
*
Control treatments were performed without nitrogen (N−) or with 25 mg of nitrogen, as NH4NO3, per jar (N+). Each treatment was composed of three replicates of two plants of L. corniculatus cv São Gabriel in each Leonard jar inoculated with one rhizobia culture (5 mL of one rhizobia culture with 108 cfu mL−1). Means followed by different letters in the same column differ by the Scott-Knott test (p < 0.01).