Table 1.
Identified proteins
| Band name | Size (kDa) on gel | Protein identification by nanoLC-ESI-MS/MS | Match to ORF [Accession number in NCBI] | Best hit in BLAST: identity % (query cover %) | Predicted size (kDa) |
|---|---|---|---|---|---|
| 1 |
≈ 55 |
Hypothetical protein |
FCOL_07765 [YP_004942163.1] |
FCOL_11410 47% (100%) |
53.5 |
| 2 |
≈ 55 |
OmpA family outer membrane protein P60 |
FCOL_09105 [YP_004942423.1] |
FP0156 Outer membrane protein precursor; OmpA family P60 [F. psychrophilum JIP02/86] 70% (100%) |
50.2 |
| 3 |
45 |
Hypothetical protein |
FCOL_02860 [YP_004941210.1] |
FP1486 Protein of unknown function [F. psychrophilum JIP02/86] 55% (98%) |
44.5 |
| 4 |
35 |
Hypothetical protein |
FCOL_08865 [YP_004942378.1] |
FP0017 Putative cell surface protein precursor SprF [F. psychrophilum JIP02/86] 68% (94%) |
37.9 |
| 5 |
20 |
Hypothetical protein |
FCOL_11765 [YP_004942947.1] |
FP2260 Protein of unknown function precursor [F. psychrophilum JIP02/86] 48% (100%) |
24.4 |
| ECP | 13 | Hypothetical protein | FCOL_04265 [YP_004941480.1] | - | 18,1 |
Protein identification of the membrane vesicle contents and the extracellular protein of Flavobacterium columnare B067 by nanoLC-ESI-MS/MS, and the subsequent identification by BLAST search. ORF annotation refers to F. columnare ATCC 49512 complete genome [NCBI: NC_016510].