Table 1.
Characteristics and performance data of the primers used for reference gene-stability measure M and qPCR
| Gene symbol | Primer sequence (forward, reverse; from 5’ to 3’) | NCBI GeneBank | Product size (bp) |
Skeletal muscle |
Liver |
||||
|---|---|---|---|---|---|---|---|---|---|
| Slope | R 2# | Efficiency* | Slope | R 2# | Efficiency* | ||||
| ACADL |
AAGGATTTATTAAGGGCAAGAAGC |
NM_012819.1 |
380 bp |
-4.45 |
1.000 |
1.68 |
-3.54 |
0.999 |
1.92 |
| GGAAGCGGAGGCGGAGTC | |||||||||
| ACADM |
CAAGAGAGCCTGGGAACTTG |
NM_016986.2 |
154 bp |
-3.29 |
0.998 |
2.01 |
-3.29 |
0.998 |
2.01 |
| CCCCAAAGAATTTGCTTCAA | |||||||||
| ACADS |
ACATCTCTTCCCCACATCGC |
NM_022512.2 |
204 bp |
-2.98 |
0.999 |
2.16 |
-3.55 |
0.999 |
1.91 |
| CCGAACTTCAGGATGGGTCC | |||||||||
| ACOX1 |
CTGGGCTGAAGGCTTTTACT |
NM_017340.2 |
172 bp |
-3.60 |
0.997 |
1.90 |
-3.97 |
0.999 |
1.79 |
| GCTGTCTGCAGCATCATAAC | |||||||||
| ALDH9 |
TTGAGCGGCTGCGACACGAC |
NM_022273.2 |
82 bp |
|
- |
|
-3.42 |
0.999 |
1.96 |
| TGACCTCGCTCCTCCGCGTA | |||||||||
| ATP5B |
GCACCGTCAGAACTATTGCT |
NM_134364 |
203 bp |
-3.58 |
0.998 |
1.90 |
-3.33 |
0.999 |
2.00 |
| GAATTCAGGAGCCTCAGCAT | |||||||||
| BBOX1 |
GGATGGGGCTCGCTTGATGCA |
NM_022629.1 |
281 bp |
|
- |
|
-3.38 |
0.997 |
1.98 |
| GGAGTCCTGCTCTGGCCTCCT | |||||||||
| CANX |
CCAGATGCAGATCTGAAGAC |
NM_172008 |
175 bp |
-3.30 |
1.000 |
2.01 |
-3.45 |
0.999 |
1.95 |
| CTGGGTCCTCAATTTCACGT | |||||||||
| MDH1 |
CAGACAAAGAAGAGGTTGCC |
NM_033235.1 |
206 bp |
-3.41 |
0.999 |
1.96 |
-3.27 |
0.999 |
2.02 |
| CGTCAGGCAGTTTGTATTGG | |||||||||
| RPL13 |
CTTAAATTGGCCACGCAGCT |
XR_086310 |
198 bp |
-3.20 |
0.999 |
2.05 |
-3.53 |
0.997 |
1.92 |
| CTTCTCAACGTCTTGCTCTG | |||||||||
| SLC22A5 |
GAACTCACGAGCCTCGCACGC |
NM_019269.1 |
117 bp |
-2.98 |
1.000 |
2.16 |
-3.51 |
1.000 |
1.93 |
| TCGTCGTAGTCCCGCATGCC | |||||||||
| TOP1 |
GAAGAACGCTATCCAGAAGG |
NM_022615 |
137 bp |
-3.45 |
0.999 |
1.95 |
-3.34 |
0.998 |
1.99 |
| GCTTTGGGACTCAGCTTCAT | |||||||||
| YWHAZ | GACGGAAGGTGCTGAGAAA |
NM_013011 | 198 bp | -3.11 | 0.999 | 2.10 | -3.30 | 0.999 | 2.01 |
| GCAGCAACCTCAGCCAAGT | |||||||||
#Coefficient of determination of the standard curve. *The efficiency was determined by [10-slope].