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. 2014 Jun 25;15(1):524. doi: 10.1186/1471-2164-15-524

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© Levy et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of rooting ability and miR156 and miR172 expression in juvenile (Juv), midphase (Mid) and mature (Mat) cuttings. Cuttings were excised 15, 45 or 200 cm above the ground (a), treated with auxin (20 sec deep in 6gr/l K-IBA) and incubated on rooting tables. (b) Percent rooting and callus formation after 30 days. Bars show averages and standard error. Bars with different letters are statistically different as determined by Scheffe analysis p < 0.05. (c,d) RNA was prepared from the bottom of the cuttings and miR156 and miR172 expression analysis was performed by Northern blots: (c) average and standard error of three experiments, asterisk show statistically significant difference as determined by Scheffe analysis p < 0.05. (d) representative gels.