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. 2014 Jun 25;15(1):524. doi: 10.1186/1471-2164-15-524

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© Levy et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Rejuvenation of E. brachyphylla and determination of miR156 and miR172 expression. (a) Sections from a 7-month-old tree, which had lost its rooting ability, rejuvenated in tissue culture (TC). (b) Rooting percentage in E. brachyphylla plants at different ages. (c,d) Analysis of miR156 and miR172 expression: (c) quantitative analysis of the gels in (d). Rooting was determined using 50 cuttings and RNA was prepared from 30 cuttings from each age.