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. 2014 Jun 25;15(1):524. doi: 10.1186/1471-2164-15-524

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© Levy et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Analysis of miR156, miR172 and their predicted targets in juvenile and mature cuttings during induction of adventitious root formation. Cuttings were excised, treated with auxin and incubated on rooting tables for the indicated times. RNA samples were prepared and the expression of microRNAs and predicted targets was determined by northern blot and nanostring analysis, respectively from three independent biological repeats. Quantitaive analysis of (a) miR156 expression, and (b) miR172 expression. (c) Representative gels: J, juvenile; M, mature. Quantitative analysis of the predicted targets of (d) miR156 and (e) miR172. Asterisks show statistically significant difference as determined by Scheffe analysis p < 0.05.