. 2014 Jul 4;15(1):553. doi: 10.1186/1471-2164-15-553

Table 5.

Comparison of RNA-seq data with expression data from qRT-PCR

NCBI gene ID	UniProt_ID	Description	Method	Expression level in ET	Expression level in NET	Fold change
840475	Swiss-Prot:Q9LP24	Leucine-rich repeat receptor-like protein kinase	RNA-seq	Log-cpm in ET	Log-cpm in NET	log2 ratio (ET/NET)
			RNA-seq	605.5	3640.6	2.6
			qRT-PCR	ET(ΔCT)	NET(ΔCT)	2^-ΔΔCT
			qRT-PCR	9.6	4.6	2.1
817958	Swiss-Prot:Q6X7J4	WUSCHEL-related homeobox 9	RNA-seq	Log-cpm in ET	Log-cpm in NET	log2 ratio (ET/NET)
			RNA-seq	155.7412756	0.353625	8.8
			qRT-PCR	ET(ΔCT)	NET(ΔCT)	2^-ΔΔCT
			qRT-PCR	3.0	1.3	3.4
831649	Swiss-Prot:Q8GY25	WUSCHEL-related homeobox 12	RNA-seq	Log-cpm in ET	Log-cpm in NET	log2 ratio (ET/NET)
			RNA-seq	136.2	1.8	6.3
			qRT-PCR	ET(ΔCT)	NET(ΔCT)	2^-ΔΔCT
			qRT-PCR	0.7	0.1	1.5

Three biological repeats were reverse transcribed and amplified independently. The raw threshold cycle (Ct) values were normalized against the WS0109_C05 standard to generate the normalized ΔCt values that were used to calculate the fold-change in expression in embryonic tissue (ET) and non-embryonic tissue (NET) of the three genotypes. Log-cpm, log-counts per million.