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. 2014 May 6;16(3):235–246.e1. doi: 10.1016/j.neo.2014.03.005

Figure 1.

Figure 1

HaCaT-ras A-5RT3 variants represent different angiogenic and invasive stages. Quantification (A and B) of immunostainings for CD31 and VEGFR2 (C) shows the highest vessel density (A and C), angiogenic activity (B and C), and invasiveness (C) in s.c. advanced tumors (n = 5), whereas i.d. tumors (n = 5) have an intermediate angiogenic and invasive stage, and s.c. early tumors are at the onset of angiogenesis and invasion (n = 7). (C) Representative immunostainings of the different HaCaT-ras A-5RT3 variants for CD31 (green), VEGFR2 (red), DAPI (blue), or PK (blue), respectively. Right panels (bar, 50 μm) are magnified selected areas of the left panels (bar, 200 μm) (indicated by the red square). Data are presented as means ± SD (*P < .05 and ***P < .001).