Figure 3.

Quantification of SMA-positive fibroblasts (A) and F4/80-positive macrophages (B) shows a differential accumulation at the distinct stages of angiogenesis and invasion. Highest accumulation of these cells is found in s.c. advanced tumors at the highest angiogenic and invasive stage. Notably, no macrophages are detected in the s.c. early tumors at the onset of angiogenesis and invasion. (C) Representative immunostainings for SMA (red), F4/80 (green), and DAPI (blue) of an s.c. early tumor at the onset of angiogenesis, an i.d. tumor at intermediate stage of angiogenesis and invasion, and an s.c. advanced, highly angiogenic, and invasive tumor. Bar, 100 μm. Data are presented as means ± SD (**P < .01 and ***P < .001; s.c. early: n = 7, i.d. intermediate: n = 5, and s.c. advanced: n = 5).