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. 2014 Apr 13;16(3):265–278.e13. doi: 10.1016/j.neo.2014.03.001

Figure 4.

Figure 4

(A) Dual-luciferase reporter assay showed that rCthrc protein inhibited Wnt/β-catenin signaling of primary GIST cells in a dose-dependent manner. The results shown are mean±SD of relative firefly/Renilla ratio. (B) Noncanonical Wnt/PCP signaling of GIST cells was activated by rCTHRC1 protein in a dose-dependent manner. (C) The inhibitory effect of rCTHRC1 on Wnt/β-catenin signaling was partially blocked by Wnt5a monoclonal neutralizing antibody. (D) The promoting effect of rCTHRC1 protein on Wnt/PCP signaling was almost blocked by Wnt5a monoclonal neutralizing antibody. (E) The promoting effect of Wnt3a on Wnt/β-catenin signaling was almost blocked by Wnt3a monoclonal neutralizing antibody. (F) The promoting effect of Wnt5a on Wnt/PCP signaling was almost blocked by Wnt5a monoclonal neutralizing antibody. (*, P < 0.05; **, P < 0.01).