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. 2014 Apr 13;16(3):265–278.e13. doi: 10.1016/j.neo.2014.03.001

Figure 5.

Figure 5

(A) Analysis of the active and total RhoA,Rac1 and Cdc42 in primary GIST cells treated with rCTHRC1 protein by pull-down assay.(B) Quantitative analysis of grey value for active Rac1/ -total Rac1 ratio using ImageJ software. (C) Quantitative analysis of grey value for active RhoA/ -total RhoA ratio using ImageJ software. (D) Quantitative analysis of grey value for active Cdc42/ total Cdc42 ratio using ImageJ software. (E) Rac1 G-LISA assay was used to assess the levels of GTP-bound Rac1 in GIST cells treated with rCTHRC1 protein. (F) RhoA G-LISA assay was used to assess the levels of GTP-bound RhoA in GIST cells treated with rCTHRC1 protein. (*, P < 0.05; **, P < 0.01).