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VASH2 expression in stably transfected MCF-7 and BT474 cells. MCF-7 cells were transfected with a vector expressing EGFP (MCF7-EGFP) or VASH2 (MCF7-VASH2); BT474 cells were transduced with scrambled shRNA (BT474-scramble) or VASH2-targeting shRNA (BT474-shVASH2). The level of VASH2 protein in each cell type was assessed by immunoblotting. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; VASH2, vasohibin-2; EGFP, enhanced green fluorescent protein; shRNA, short hairpin RNA.
