Figure 2.

Short-term treatment with low-dosed temsirolimus differentially alters adhesion and migration of resistant and nonresistant RCC cells. The resistant tumor cells were treated with fresh medium (without temsirolimus) for 3 days and then exposed to 10 nM temsirolimus. Medium change followed by 10 nM temsirolimus treatment was also carried out with the drug-sensitive cell lines. A shows time-dependent RCC adhesion to HUVEC (representative for KTC^par and KTC^res), B shows the collagen and fibronectin binding assay, and C demonstrates chemotactic behavior of the tumor cell sublines evaluated by the Transwell chamber assay (60-minute values). Percentage is related to controls not treated with 10 nM temsirolimus, set to 100%. Each diagram represents one of six experiments. * indicates significant difference to the temsirolimus-free control. # indicates significant difference between the resistant and the sensitive RCC cell line.