Figure 5.
Elevated MMP expression in resistant cells. Quantitation of MMP expression in sensitive and resistant cells grown in physiological or acidic pH media for 24 hours. Transcripts were normalized to β-actin expression before analysis. (A) Ratio of expression of MMP-2, -3, -9 and -13 in LL/2R and PC3MS cultured in acidic media relative to cells cultured in physiological media. (B) Ratio of expression of MMP-2, -3, -9, and -13 in LL/2R cells relative to expression in PC3MS cells cultured in acidic and physiological media. Data are the average of three independent experiments and is reported as mean ± SD. *P < .05. R, resistant; S, sensitive.