Supplemental Figure 2.

Metabolic analysis of buffer therapy resistant and sensitive cells. A. Line graph showing sensitive and resistant cells oxygen consumption rates (OCR) through a mitochondrial stress test. Five basal measurements were obtained while cells were in media containing 5.55 mmol/l glucose, 2 mmol/l glutamine and 1 mmol/l pyruvate. Treatment of cells with oligomycin (1 μmol/l) led to a decrease in OCR that can be related to ATP production, followed by an increase in OCR to maximal respiration rates after treatment with FCCP (1 μmol/l). Finally, complete OXPHOS inhibition was achieved after treatment with Rotentone (1 μmol/l) and Antimycin A (1 μmol/l) B. Line graph showing sensitive and resistant cells extracellular acidification rates (ECAR) through a glycolytic stress test. Five basal measurements of cells that had been glucose starved for 2 hours shows non-glycolytic acidification. Stimulation of cells with glucose (5.55 mmol/l) results in basal glycolysis levels, which is increased to maximal glycolysis flux upon treatment with oligomycin (1 μmol/l). Glycolysis inhibition is achieved with treatment of 2DG (100 mmol/l). Data was normalized to mg protein and is reported as mean ± SD. _R= Resistant, _S= Sensitive.