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. 2014 May 24;16(4):354–364.e3. doi: 10.1016/j.neo.2014.04.005

Supplemental Figure 3.

Supplemental Figure 3

Metabolic profile analysis of HCT116-Luc and HCT116-GFP cells. Glycolytic and mitochondrial stress tests show different metabolic profiles of HCT116-Luc and HCT116-GFP cells. (A) Extracellular acidification rates (ECAR) of cells after stimulation of glycolysis with glucose (5.55 mmol/l), oligomycin (1 μmol/l) and glycolysis inhibitor, 2DG (100 mmol/l). (B) Oxygen consumption rates (OCR) of cells before treatment with oligomycin (1 μmol/l), FCCP (1 μmol/l) and Rotentone (1 μmol/l) and Antimycin A (1 μmol/l). (C) OCR/ECAR ratio of cells during basal metabolism. (D) Representative brightfield microscopy images of HCT116-Luc and HCT116-GFP cells in pH 7.4 culture conditions. Data shown as mean ± SD. Scale bars represent 100 μm.