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. 2014 May 24;16(4):365–374.e2. doi: 10.1016/j.neo.2014.04.004

Figure 3.

Figure 3

NHERF1 apical PM localization is required for 3D gland development. (A) Immunoblot with indicated antibodies of whole lysates (30-μg proteins) from cells expressing Myc-tagged NHERF1 wild type, double PDZ domain mutant (PDZ1-2-DM), and ERM-binding–deficient ΔSNL mutant is shown. (B) Immunoblot as in A shows collapse of phosphorylated forms (bracket) to a single form (arrowhead) following CIP treatment. (C) IF analysis of early gland development from Caco-2 cells expressing the diagrammed NHERF1 and mutants. Arrowheads indicate NHERF1 expression at the apical PM.