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. 2014 May 24;16(4):365–374.e2. doi: 10.1016/j.neo.2014.04.004

Figure 4.

Figure 4

Differential roles of ezrin and moesin in gland morphogenesis. (A) IF analysis with NHERF1 and ezrin (left) or moesin (right) antibodies of early gland formation shows colocalization of both ERMs with NHERF1 at the apical PM. (B) Western blot of proteins from Caco-2 cell lysates shows shRNA knockdown of ezrin (shE No. 8) and moesin (shM No. 4) and corresponding GAPDH-normalized levels (%). (C and D) Confocal IF of moesin-depleted (shMoesin) (C) and ezrin-depleted (shEzrin) (D) mature structures shows disrupted gland morphogenesis only by moesin depletion.