Figure 6.

Parallelism between morphologic changes occurring in the CRC transformation paradigm and in 3D glands by NHERF1 apical PM loss. Hematoxylin and eosin staining of a CRC resection specimen (original magnification, × 200) shows areas of normal mucosa, tubular adenoma, and high-grade dysplasia, the latter showing cribriform architecture (arrows), mitoses (arrowhead), nuclear enlargement and pleomorphism, and complete loss of cell polarity. The development of 3D spheroids by NHERF1 depletion reproduces high-grade dysplasia and validates the 3D intestinal gland system.