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Akt localizes at the basal cell membrane in 3D Caco-2 glands. A. Schematic drawing of a 3D gland with the polarized distribution of phosphoinositides. B. Western blot of whole cell lysates (30 μg proteins) showing expression of HA-tagged Akt and vector control in Caco-2 cells. Endogenous Akt is indicated. C. Confocal IF analysis of Caco-2 3D glands with HA and NHERF1 antibodies. Note basal membrane distribution of HA-Akt(arrow).
