Figure 1.

α-Synuclein is overexpressed in SH-SY5Y-Syn cells. (a) RT-qPCR analysis of α-synuclein overexpression in SH-SY5Y-Syn cell lines after serial passages, compared to β-actin. (b)–(d) Western blot (b) or immunofluorescence analysis ((c) and (d)) indicated α-synuclein overexpression in protein level in serially propagated SH-SY5Y-Syn stable cell line; results were normalized to β-actin. The GFP-positive cells overexpressed α-synuclein. Statistical significance was showed as **P < 0.01, ns: no significance.