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Factors and levels of central composite design for amino acid, nucleoside analogue, and time.
| Symbol | Variables | Code level | ||||
|---|---|---|---|---|---|---|
| −1.6818 | −1 | 0 | 1 | 1.6818 | ||
| A | Hypoxanthine (g/L) | 0.53 | 1 | 5 | 9 | 10.53 |
| B | L-alanine (g/L) | 5.27 | 8 | 12 | 16 | 18.72 |
| C | Culture time (days) | −0.09 | 4 | 10 | 16 | 20.09 |
