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In silico restriction pattern of fliI gene (640 bp) specific to H. pylori digested with Mnl I. The virtual gel was generated by interpolating experimental data using NEB cutter V2.0. KC525441 (GenBank accession number) represents in silico restriction pattern like J99; KC525439, KC525440, KC525442, and KC525443 produced varying restriction pattern. I*: restriction pattern of J99; W**: wild type restriction pattern; A, B1, and B2: mutated/changed restriction pattern of Mnl I.
