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(A) Arrangement of experimental and control siRNA duplexes in a secondary assay for ubiquitin modifiers that control the nuclear translocation of NF-κB. (B) Graphical overview of the nuclear accumulation of NF-κB as a number of standard deviations from control non-silencing siRNA duplexes in HeLa or A549 cells treated with the respective siRNA duplexes and TNF for the indicated periods.
