Figure 4.
Disease phenotype of SlMKKs-silenced plants after inoculation with B. cinerea. Disease symptom (A) and lesion size (B) in selected leaves of the TRV-SlMKKs- and TRV-GUS-infiltrated plants in detached leaf inoculation assays. Botrytis inoculation was done by dropping spore suspension (1 × 105 spores/mL) on detached leaves of tomato plants and lesion sizes were measured at 3 days after inoculation on a minimum of 20 leaves in each experiment. At least ten leaves from ten individual silenced or control plants were used for each experiment. Data presented in (B) are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level.