Table 1.
Primers used in this study for different purposes
| Primers | Sequences (5′-3′) | Size (bp) |
|---|---|---|
|
Cloning of cDNA | ||
| SlMKK1-F |
ATGAAGAAAGGATCTTTTGCAC |
1074 |
| SlMKK1-R |
TTATAGCTCAGTAAGTGTTGCC |
|
| SlMKK2-F |
ATGCGACCAGCCGCCAACTCCA |
1080 |
| SlMKK2-R |
TCAAGAAGAGGAGGAAAAATGA |
|
| SlMKK3-F |
ATGAAGACGGCGAAGCCATTGA |
1065 |
| SlMKK3-R |
TTATCTTGGAAAATTTACTGGG |
|
| SlMKK4-F |
ATGGCCTTAGTTCGTGATCGCC |
1008 |
| SlMKK4-R |
TTAGGTGGATTTCAAATCGATA |
|
| SlMKK5-F |
ATGGCTGGACTGGAGGAATTG |
1548 |
| SlMKK5-R |
CTATTGAGTAATGAAAAGTTC |
|
|
VIGS constructs | ||
| SlMKK1-VIGS-F |
TGC TCTAGAGCAAAACCCCATTTGCCTGA |
360 |
| SlMKK1-VIGS-R |
CCG GAGCTCCTGATGGCTGTATAACTGAA |
|
| SlMKK2-VIGS-F |
TGC TCTAGACAATCCAATACCATTATTCA |
360 |
| SlMKK2-VIGS-R |
TCCCCCGGGAAGACGGACAGAATCCTCGT |
|
| SlMKK3-VIGS-F |
TGC TCTAGAGGGTTATTTGGGCGGTCCGT |
360 |
| SlMKK3-VIGS-R |
CCG GAGCTCAATGTTCCAACCCATTTATG |
|
| SlMKK4-VIGS-F |
TGCTCTAGA ACTTGAAAAGCTTAAGGTTC |
400 |
| SlMKK4-VIGS-R |
CCGCTCGAG ACGATTCACTAAAAGGTTCG |
|
| SlMKK5-VIGS-F |
TGC TCTAGAATGGCTGGACTGGAGGAATT |
360 |
| SlMKK5-VIGS-R |
CCG GAGCTCCTGCCTTTTCTCCTTCTCAA |
|
|
Transient expression | ||
| SlMKK2DD-GFP-F |
GCTGTACAAGGGATCCATGCGACCTCTTCAACCACC |
1080 |
| SlMKK2DD-GFP-R |
TAATTAACTCTCTAGATTAAGAAGAAAAATGAGGAG |
|
| SlMKK4DD-GFP-2F |
TGCTCTAGAATGGCCTTAGTTCGTGATCGCCG |
1008 |
| SlMKK4DD-GFP-2R |
TCCCCCGGGTTAGGTGGATTTCAAATCGATAC |
|
|
qRT-PCR | ||
| SlMKK1-RT-F |
GGCCAATACCTTTGTCGGCACATA |
148 |
| SlMKK1-RT-R |
TCCCTCGGGTGGTTTATATGGGAA |
|
| SlMKK2-RT-F |
AAGGTTCTACATCGTCCCACTGGA |
100 |
| SlMKK2-RT-R |
TCTCGATCTCACGGCACATCTGAA |
|
| SlMKK3-RT-F |
AATGCTAGCCAGCTCTATGGGTCA |
181 |
| SlMKK3-RT-R |
AGCTTGCTGGTCCTCTGACTGTAT |
|
| SlMKK4-RT-F |
CGCTAAGCAAGTGCTTGGTGGATT |
158 |
| SlMKK4-RT-R |
TGCAAGGATCCAAAGTCCTTCCCA |
|
| SlMKK5-RT-F |
CCAGAACCTATCCTTTCCTCAAT |
103 |
| SlMKK5-RT-R |
GATTTGCTGGCTTTATGTCTCTG |
|
| SlActin-RT-F |
CCAGGTATTGCTGATAGAATGAG |
113 |
| SlActin-RT-R |
GAGCCTCCAATCCAGACAC |
|
| SlPI I-RT-R |
GTTGTACAAATGCCTGTGGTGAC |
135 |
| SlPI I-RT-R |
GGTAAGAGTACATGAAGAGATGC |
|
| SlPI II-RT-F |
CATCTTCTGGATTGCCCA |
106 |
| SlPI II-RT-R |
ACACACAACTTGATGCCCAC |
|
| SlLapA-RT-F |
GGGACTAATGATGTTTGGAA |
109 |
| SlLapA-RT-R |
GTGGCAATTTTATTTAGGCA |
|
| SlPR1b-RT-F |
TTTCCCTTTTGATGTTGCT |
96 |
| SlPR1b-RT-R |
TGGAAACAAGAAGATGCAGT |
|
| SlPR-P2-RT-F |
CGATCTAAATTGATTTCATAGTACG |
116 |
| SlPR-P2-RT-R |
TCGTGAAGGATATACAAAATACA |
|
| BcActin-RT-F |
CGTCACTACCTTCAACTCCATC |
107 |
| BcActin-RT-R |
CGGAGATACCTGGGTACATAGT |
|
| NbActin-RT-F |
ACCAGATTAATGAGCCCAAGAG |
97 |
| NbActin-RT-R |
CCAACAGGGACAGTACCAATAC |
|
| NtPR1-RT-F |
CCGTTGAGATGTGGGTCAAT |
100 |
| NtPR1-RT-R |
CGCCAAACCACCTGAGTATAG |
|
| NtPR2-RT-F |
CAACCCGCCCAAAGATAGTA |
98 |
| NtPR2-RT-R |
TGGCTAAGAGTGGAAGGTTATG |
|
| NtPR4-RT-F |
GGATGATGTTGACAGCAGAGA |
116 |
| NtPR4-RT-R |
GTAGGACACGAGGTAGGTATCA |
|
| NtPR5-RT-F |
GCTCGATTACGTCTTGTCTCTC |
104 |
| NtPR5-RT-R | CTCTAGCATGGTGGATTGACTT | |