Table 2.
Predicted knockout (MOMA) and overexpression (FSEOF) targets for increased production of hSOD.
| Genes | Enzyme names | Gene name | log2flux change hSOD/X-33 | |
|---|---|---|---|---|
| MOMA target | ||||
| PIPA03164 PIPA01726 | Pyruvate decarboxylase | PDC1 | ⪡ −5 | |
| PIPA03313 PIPA02544 | Alcohol dehydrogenase (ethanol) | ADH2 | ⪡ −5 | |
| PIPA00390 | Aldehyde dehydrogenase (acetylaldehyde, NAD) | ALD4 | n.d.⁎ | |
| PIPA02794 PIPA03785 PIPA04299 PIPA03623 | Pyruvate dehydrogenase | PDA1 | 0.23±0.02 | |
| PIPA03441 | Triose-phosphate isomerase | TPI1 | 0.00±0.03 | |
| PIPA02567 | Glycerol-3-phosphate dehydrogenase (FAD) | GUT2 | 0.00±0.03 | |
| FSEOF target | ||||
| PIPA08178 | Glucose 6-phosphate dehydrogenase | ZWF1 | 0.04±0.23 | |
| PIPA04435 | 6-phosphogluconolactonase | SOL3 | 0.04±0.23 | |
| PIPA03124 | Phosphogluconate Dehydrogenase | GND2 | 0.04±0.23 | |
| PIPA03251 | Ribulose 5-phosphate 3-epimerase | RPE1 | −0.15±0.02 | |
| PIPA02093 | Transketolase | TKL1 | 0.09±0.22 | |
| PIPA03744 | Transaldolase | TAL1 | 0.09±0.22 | |
| PIPA06084 | Glycerol-3-phosphate dehydrogenase | GPD1 | 0.01±0.02 | |
| PIPA02244 | Malate dehydrogenase | MDH1 | 0.56±0.01 | |
| PIPA03564 | Glutamate dehydrogenase (NADP) | GDH3 | n.d.* | |
⁎
Not determined.