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. 2014 Jun 23;16(3):R131. doi: 10.1186/ar4588

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Copyright © 2014 Gramoun et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Photomicrographs of Prussian blue and CD68-immunostained sections confirming in vivo superparamagnetic iron oxide nanoparticles(SPION) labeling of macrophages.(A) Double-stained photomicrographs of (i) Prussian-blue-stained SPIONs (ii) immunofluorescent-stained macrophages using mouse anti-rat CD68 1^ry antibody (as a marker of macrophages) and anti-mouse fluorescein isothiocyanate (FITC) second antibody at 20 times magnification (iii) the overlay of the two previous images. (B, C) Photomicrographs of two separate examples of (i) Prussian blue followed by (ii) CD68-immunostained sections at 40 times magnification. Sections were stained consecutively and micrographs of the same region of interest were obtained.